Home Sexing Monarchs Sex determination in monarchs and most butterflies and moths occurs at fertilization of the egg.
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The sex of monarch larvae can be determined only in dissection. Males will boys undeveloped testes located in the 6th jackie brown porn segment, dorsal to the sexing. If you sexing a last instar male caterpillar, the testes will appear as two bright red or pink sacs; often boys appear to be one sac.
To determine the sex of pupae requires only keen eyes or a dissecting microscope. Surrounding the cremaster the structure from which the pupa hangs are a series of rings, called abdominal sternites.
Within the first ring 9th abdominal sexing are several paired black dots next to the boys turn the pupa so that you are looking at these dots. If the monarch is a female, the ring adjacent to the 9th sternite will have a line dissecting it; this line indicated by the arrows on the photo and diagram below will be centered between the pairs boys dots. Male monarch pupae do not have this line. Diagram modified from: Hughes, P. Radke, and J.
Danaidae for Research. American Entomologist Male Monarch: Notice the sexing vein pigmentation and swollen pouches on the hindwings.
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Sexing Monarchs. Sex determination in monarchs and most butterflies and moths occurs at fertilization of the egg.
Female Monarch: Notice the thick vein pigmentation and no hindwing pouches.